Today, HPLC continues to evolve rapidly toward higher speed, efficiency, and sensitivity, driven by needs of life sciences and pharmaceutical applications. Pharmaceutical products formulated with more than one drug, typically referred to as combination products the development and validation of analytical methods.
. There are different modes of separation in HPLC Normal phase mod, Reversed phase mode, Ion exchange chromatography, Reverse phase ion pair chromatography, Affinity chromatography and Size exclusion chromatography, Spectrophotometric, High performance liquid chromatography (HPLC) & High performance thin layer chromatography (HPTLC)] for drug products containing more than one active ingredient. This method of analysis is gaining importance due to simple, rapid, precise, highly accurate and less time consuming The spectrophotometric analysis of drugs rarely involves the measurement of absorbance of sample containing only one absorbing component. Liquid chromatography (LC) is a physical separation technique conducted in the liquid phase.
High-performance liquid chromatography (HPLC), sometimes called high-pressure liquid chromatography. Normal phase mode of separation is therefore, not generally used for pharmaceutical applications because most of the drug molecules are polar in nature and hence take longer time to elute.
Key word: HPLC, Drug, analytical, mode of searation, chromatography, spectrophotometric.