Background: The plant Gmelina arborea has been traditionally used in India for several medicinal purposes like anthelmintic, diuretic, antibacterial, hepatoprotective, anti-inflammatory, antioxidant and antidiabetic. It contains phytoconstituents like alkaloids, carbohydrates, anthraquinone glycosides, gums, mucilages, tannins, phenolic compounds and flavonoids. Aims: The objective of present study is to isolate a compound from ethanolic extract of G. arborea and to explore the anti-inflammatory activity of isolated compound.
Material and methods: The isolation of compound was done by column and thin layer chromatographic methods. The isolated compound was characterized to elucidate its structure by spectroscopic methods like ultraviolet, infrared, nuclear magnetic resonance and mass spectroscopy. The anti-inflammatory activity of phytoconstituent was evaluated by Carrageenan-induced rat paw edema test method using Wistar rats as animal model. Statistical analysis used: All data are verified for statistically significant by using one way ANOVA at 5 % level of significance (p < 0.05). Results: A flavonoidal compound was isolated as yellow colour crystal with melting point 177±1 °C with molecular formula C16H15O5 and IUPAC name 5,7-dihydroxy-4-methoxy flavone. The compound was able to show good anti-inflammatory activity in comparison with standard drug Diclofenac sodium. Conclusion: It could be concluded that the isolated compound is a flavonoid and it possess anti-inflammatory activity.
Key words - Gmelina arborea, Flavonoid, Spectroscopy, Carrageenan, Inflammation