Objective: The study aims to determine the anti-inflammatory activity of cream containing semi-purified flavonoids of Limnophila sessiliflora (ambulia) leaves in Rattus norvegicus (Sprague Dawley Rats) using three concentrations namely: 1%, 2%, and 4%.
Methodology: Limnophila sessiliflora leaves were macerated for three days and were further extracted to obtain the semi-purified flavonoids. After semi-purification, the extract was subjected to various tests to confirm the presence of flavonoids. Then, it was subjected to cream formulation and anti-inflammatory determination. Physical tests of the cream, followed by Dermal Irritation Test on rats, were also conducted prior to formulation and anti-inflammatory testing.
Results: The semi-purified extract of Limnophila sessiliflora (Ambulia) resulted positive to flavonoids. Also, results showed that the cream containing semi-purified flavonoids met the Physical Tests requirements; did not exhibit dermal irritation; and exerted only a minimal anti-inflammatory effect that is not comparable to the reference standard, Indomethacin.
Conclusion: The cream containing the semi-purified flavonoids of Limnophila sessiliflora did not exhibit an anti-inflammatory activity up to 4% of concetration.
Keywords: Limnophila sessiliflora, semi-purified flavonoid, cream, anti-inflammatory