A simple, precise and economical spectrophotometric method has been developed for the estimation of Ezogabine in pharmaceutical formulation. Ezogabine (EZG) is an anticonvulsant drug used for the treatment of partial epilepsy. EZG are available in tablet dosage form in a dose of 50 mg, 200 mg and 400 mg. The purpose of present study is to develop a spectroscopic method for estimation of EZG in tablet formulation.
The method is based upon estimation of EZG from pharmaceutical formulation by standard curve method. The absorbance was measured at 218.5 nm. The accuracy and reproducibility results are close to 100% with RSD<2. In proposed method precision was studied as repeatability (RSD<2) and inter and intra- day variations (RSD<2) shows the high precision of the method. The LOD for EZG was found to be 1.047μg/mL. The LOQ for EZG was found to be 3.17μg/mL. The results of proposed method have been validated as per ICH guidelines.
Keywords: Ezogabine, Accuracy, Precision, LOD, LOQ, ICH guidelines.