Abstract 01 V2 I5

Siddha system of medicine pioneering in emphasizes the biological activity of the various phytocomponents with respect to the etiology and pathophysiology of various dreadful diseases emerging in humans and animals. It is evident that there are some medicinal plants used in siddha has potency of acting as an anesthetics, analgesics, anti-microbial, immune modulators, Hepato , neuro and nephron protectant etc.

But the most critical scenario is that information about most of the novel preparations is now extinct due to lack of proper documentation and standardization. Hence the main aim of the present investigation is to standardize the formulation Oma Legium (OL) as per AYUSH guideline and to reveal the property of the formulation to the scientific community for better understanding about the standards of the formulation. The results obtained from standardization and physiochemical analysis clearly reveals that total ash value of OL is about 5.66%, Loss on drying 39.43%, Total reducing sugar 22.76% and Total fat content 6.75%.

The result of the phytochemical analysis indicates that the formulation OL shows the presence of alkaloids, flavonoids, glycosides, coumarins, triterpenoids, phenols, tannins, saponins, betacyanins, proteins and carbohydrates. HPTLC finger printing analysis of OL indicates the presence of 14 significant peaks with Rf value ranging from 0.01 to 0.86. From the results of the present investigation it was concluded that the formulation OL is highly sterile, safe and also possess biologically significant phytocomponents which is may be used as an ailment for treating various disease.

Keywords: Siddha system, Oma Legium, Standardization, Physiochemical, AYUSH, HPTLC,