The objective of the present study is to determine possible bioactive compounds of the different extract fractions of entire plant of Nyctanthes arbortristis linn. The present research work was carried out by using GC-MS analysis, while mass spectra of the compounds found in the extract fraction was conducted by using the database of national institute of standard and technology (NIST) having more than 62000 patterns. Forty components from different extract fraction of entire plant were identified.
Active principles with their retention time, area, height of peak, percentage of area of peak were obtained. The research reveals the potential of entire plant of Nyctanthes arbortristis Linn as a good source of bioactive such as fatty acid esters, alcohol, hydrocarbon, aldehyde, ketones, amide, terpenes, sterols that justify used of this plant for its various ailments for traditional practitioners.Keywords: GC-MS, Nyctanthes arbortristis linn, Phytol, Alpha-Comphenol, Extraction, Furfural.