Abstract 07 V2 I3

Objectives: This study aimed to determine the cytotoxic property of the fucoidan extract of Sargassum serratifolium C. Agardh using MTT assay, Allium cepa (Onion) genotoxicity test, and Artemia salina (Brine shrimp) lethality test.

Methodology: The ethanolic extract of Sargassum serratifolium C. Agardh (Aragan) was subjected to phytochemical screening prior to cytotoxicity determination. Then, the ethanolic extract was further extracted to produce a semi-purified fucoidan extract of Sargassum serratifolium C. Agardh (Aragan). The semi-purified fucoidan extract underwent Fourier Transform Infrared Spectroscopy (FT-IR) to determine the presence of sulfated polysaccharides which gives way to its cytotoxic activity. Determination of cytotoxic property of the fucoidan extract was done using MTT assay, Allium cepa (Onion) genotoxicity test, and Artemia salina (Brine shrimp) lethality test.

Findings: Preliminary phytochemical testing of the plant extract resulted positive to flavonoids, alkaloids, reducing sugars, and fixed oils. The MTT assay result showed that the fucoidan extract from S. serratifolium (Aragan) did not illicit any cytotoxic activity to MCF7 (Human Breast Cancer) cell line up to 100ug/mL concentration. Also, the Allium cepa (Onion) genotoxicity test showed that there is a minimal effect in the growth inhibition of the root tips of the onion. Lastly,  the Artemia salina (Brine shrimp) lethality test showed that fucoidan extract has a significant difference with the negative control, 1% DMSO with artificial seawater but it is not comparable to the positive control, 2% ethyl alcohol.

Conclusion: Sargassum serratifolium C. Agardh (Aragan) does not possess any cytotoxic activity in MCF7 (Human Breast Cancer) cell line up to 100ug/mL.

Keywords: Sargassum, Cytotoxic, MTT, Genotoxicity, Lethality.