Current advancement in drug discovery technology and search for novel chemical diversity have intensified the efforts for exploring lead from “Ayurveda” the traditional system of medicine in India. Orange (Citrus sinensis) is an herbaceous member of the family Rutaceae. These are native to tropical and subtropical regions of India, China, Northern Australia and New Caledonia. Clinical trials and animal research support the use of orange peel for antityphoid, antidiabetic, anticarcinogenic, antiproliferative, antioxidant, antibiotic and antifungal activity.
The major biochemical constituents of orange peel are identified as N-decylaldehyde-methyl ester of anthranilic acid, polymethoxy flavones, nobelitin, limonene, hesperidine etc. In our present investigation we have isolated major phytochemical markers from methanolic extracts of peel of orange and characterized the isolated phytochemical markers by analytical methods. Antitubercular activity of the marker (OPE-1) obtained from the methanolic extract of Citrus sinensis peel was evaluated against Mycobacterium tuberculosis by ALAMAR blue assay method (MABA), using different solvent at varying concentration. Only water soluble fractions showed significant sensitivity.
Key words: Citrus sinensis peel, Column chromatography, Antitubercular activity.