A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for Bromazepam in Bulk and Pharmaceutical tablet Formulation. Isocratic elution at a flow rate of 1ml/min was employed on symmetry Kromasil C18 column 250 x 4.6 mm ID with 5 m at ambient temperature. The mobile phase consisted of Acetonitrile: 1% Acetic acid : Water(25:45:30 v/ v/v). The UV detection wavelength was 238nm and 20 µl sample was injected. The run time for Bromazepam is 6 min.
The flow rate was found to be 1.0ml/min. The percentage recovery of the method was found to be 109%. The LOD and LOQ for Bromazepam were found to be 5µg/ml and 15µg/ml respectively. The method was successfully applied for routine quality control analysis of pharmaceutical formulation. The HPLC method can be successfully applied for the routine quality control analysis of Bromazepam formulations, which could be the better choices compared to the reported methods of literature.
Key Words: Bromazepam, Rp- HPLC, UV detection, Recovery, Precise.