Holarrhena antidysenterica (Linn.) Wall Family Apocynaceae is also known as connessi bark in English, kutaja in Sanskrit, kura or kurchi in Hindi. Its bark and seeds have been used in the treatment of dysentery and diarhhoea, anemia, epilepsy, stomach pain and cholera. In this study, toxicity profile of the plant extract on repeated administration was studied in male and female rats. The rats were treated with doses at three different levels (150, 450 and 1350 mg/kg) of the hydromethanolic extract of stem bark of the plant orally for 28 days.
During the study period, the animals were observed for signs of morbidity and mortality along with behavioral changes, effect on body weight and food and water intake. After the completion of treatment, blood was collected retro-orbitally and then the animals were sacrificed after euthanasia for gross morphology and tissues were removed for histopathological studies. Study revealed that there were no adverse or toxic effects in treated animals. The extracts did not alter the structure and function of any organ or tissue as inferred from various laboratory investigations. In conclusion, Holarrhena antidysenterica hydromethanolic stem bark extract was found to be safe up to 1350mg/kg dose in male and female rats.
Key words: Holarrhena antidysenterica stem bark, hydromethanolic extract, and sub-acute toxicity.