Eco friendly and cost effective methods of green mediated synthesis of nanoparticles are the present research in the limb of nanotechnology. The present work leads to the synthesis of silver nanoparticles from 1 mM AgNO3 solution through various concentration of aqueous leaf extract of Azadirachta indica reducing as well as capping agent. The characteristics of silver nanoparticles were studied by using UV-VIS spectroscopy, SEM and EDX. Ultraviolet scanning spectroscopy was used to detect the distinct absorption spectrum of silver nanoparticles. The peak value observed at 435 nm.
The EDX spectrum of the silver nanoparticles confirmed the presence of elemental silver signal. The size of synthesized silver nanoparticle was 146 nm. Green synthesized silver nanoparticle showed zone of inhibition against isolated Gram positive (Micrococcus, Bacillus and Staphylococcus species) and Gram negative (Klebsiella species and E.coli) bacteria. Based on the result obtained it can be said that the plant resources can efficiently use in the production of silver nanoparticle and it could be utilized in various fields in biomedical and nanotechnology.
Key words: Nanoparticles; Neem Leaves; Antibacterial Activity; SEM, EDX