Abstract 12 V1 I2

A simple, sensitive, spectrophotometric method in UV region has been developed for the determination of Pantoprazole in bulk and tablet dosage form. The method have been developed and validated for the assay of Paracetamol using Methanol and water as diluents. Solution of Pantoprazole in Methanol shows maximum absorbance at 267 nm in zero order spectrum method (method A), in first order derivative spectra (method B) show sharp peak at 258 nm when n=1, and in method C calculation of area under curve (AUC) for analysis of Pantoprazole in wavelength range 262-272nm.

The drug followed the Beer-Lambert’s law in the concentration range of 2-10 µg/ml. Result of analysis were validated statically and were found to be satisfactory. All the parameters of the analysis were chosen according to ICH [Q2 (R1)] guideline.

Keywords: Pantoprazole sodium sesquihydrate, Zero order, First order, Area under order (AUC).