RP-HPLC method was developed and validated for the estimation of Glipizide in marketed formulation (tablet). The proposed RP-HPLC method utilizes a Symmetry C18 (4.6 x l5 mm, 3.5 µm) or equivalent column, mobile phase comprising of 20 mM potassium dihydrogen phosphate:acetonitrile in the ratio of 40:60 v/v and a flow rate of 1.0 ml per minute with UV detection at 233 nm.
The retention time was found to be 2.82 minutes for Glipizide. The linearity was in the concentration range of 10-50 µm/ml (r2=0.999). The % mean recovery was found to be 100.7 % w/w. The proposed method was found to be specific, linear, accurate, precise, rugged and robust. Hence the RP-HPLC method developed and validated can be used routinely for the estimation of Glipizide in the marketed formulation (tablet).
Keywords: Glipizide, RP-HPLC, Potassium dihydrogen phosphate, Acetonitrile.