Abstract 4 V1 I1

The present investigation was aimed to evaluate antimicrobial activity of various extracts of leaves and stems of Vitex negundo Linn. by Agar Cup method. The extracts of the plant (Vitex negundo Linn.) under investigation, were separately suspended in DMSO. The activity was compared with the standard Ampicillin (antibacterial) and Fluconazole (antifungal).

Petroleum Ether extracts of leaves and stems showed more significant antimicrobial activity against tested species and comparable with standard. Transdermal hydrogel was prepared by cold fusion method with carbapol 940 and evaluated for its physiochemical properties and in-vitro diffusion studies. Promised hydrogels were also subjected to in-vitro anti-microbial evaluation. It was found both of them possessed significant antimicrobial activity and may serve as promising antimicrobial gel formulations.

Keywords: Antimicrobial activity, Microbial evaluation, Hydrogels, phytomedicine.