Glucose isomerase (GI, EC 18.104.22.168) a successful commercial enzyme used to convert D-glucose to D-fructose. Here media for GI production was optimized using design of experiments (DoE) study. The yeast extract, sucrose and MgSO4.7H2O were found to be the most critical parameter for the production of GI through OVAT screening. It has been interpreted that yeast extract, an enriched nitrogen source and sucrose, a cheap carbon source were responsible for the built up of biomass MgSO4.7H2O acted as a stimulator, which ensures more catalytic production of GI.
A central composite rotatable design (CCRD) of RSM was used to correlate the interactive effect among these variables with GI activity. Optimal media composition was found to be as: (in g L-1) yeast extract at 12.4, sucrose at 33.4 and MgSO4.7H2O at 1.0 that gave better activity 4386 U L-1 which was 2.5 fold more than basal media 2053U L-1.
Keywords: Actinoplanes missourensis, Glucose isomerase, mediaoptimisation, Response Surface Methodology.