Abstract 01 V1 I2

Background: Alzheimer’s disease (AD) is characterized by the accumulation of amyloid plaques and neurofibrillary tangles accompanied by cognitive dysfunction affecting the quality of life of patients. Objective: to investigate the effects of MSCs and/or milk Kefir grains in Alzheimer’s disease induced in female albino rats.

Materials and Methods: sixty female albino rats were divided into equal six groups (ten rats each): group1: healthy control; group 2: LPS-induced AD; group 3: LPS- induced AD in rats received single intravenous injection of MSCs; group 4: LPS-induced AD in rats received milk Kefir; group 5: LPS-induced AD in rats which received asingle intravenous injection of MSCs with a daily milk Kefir grain administration for a month; group 6: the rats of this group received kefir for one week prior to the induction of AD followed by a single intravenous Infusion of MSCs with a daily milk Kefir grain administration for a month.

AD was assessed by T maze behavioral test month after induction. Brain tissue was collected for monitoring BDNF, Bax, Bcl-2and seladin-1 gene expression with the measurement of TNF-α, IL-10 and tissue cholesterol. Plasma lipid profile, GSH and MDA were also determined.Results: revealed that significant elevation of lipid profile and oxidative stress in association with LPS-induction. BDNF, Bcl-2and seladin-1 gene expression were significantly reduced in AD while Bax mRNA gene was significantly increased. Administration of Kefir and /or MSCs suppressed LPS –induced AD. Conclusion: the pre and co-administration of Kefir with MSCs can function as a potent modulator attenuating the underling pathological process which results in progressing of brain damage.